Journal: MedComm

Article Title: The PIEZO1/CISD1/IP3R Mechanotransduction Axis Drives Macrophage Mitochondrial Dysfunction and Inflammatory Activation in Pulmonary Fibrosis

doi: 10.1002/mco2.71080

Figure Lengend Snippet: Knockdown of CISD1 improves BLM‐induced abnormal polarization of macrophages. (A) Following AAV9‐mediated knockdown of CISD1 in mice, pulmonary fibrosis was induced using BLM. Immunofluorescence staining was performed to detect expression of the MPTP protein CypD in pulmonary macrophages, scale bar = 20 µm. (B and C) Flow cytometry detection of polarization of pulmonary macrophages in each group, n = 6 biological replicates. (D and E). Flow cytometry detection of changes in the proportion of pulmonary macrophage subsets in each group of mice, n = 6 biological replicates. (F) Immunofluorescence staining detection of M1 polarization in pulmonary macrophages in each group of mice, scale bar = 20 µm. (G) Immunofluorescence staining detection of M2 polarization in pulmonary macrophages in each group of mice, scale bar = 20 µm.

Article Snippet: Note that 21 days post intratracheal administration of AAV9 virus (PackGene Biotech, Guangzhou, China) containing shNC or shCISD1, a BLM‐induced pulmonary fibrosis model was established, and the mice were sacrificed after an additional 21 days of housing.

Techniques: Knockdown, Immunofluorescence, Staining, Expressing, Flow Cytometry

Journal: MedComm

Article Title: The PIEZO1/CISD1/IP3R Mechanotransduction Axis Drives Macrophage Mitochondrial Dysfunction and Inflammatory Activation in Pulmonary Fibrosis

doi: 10.1002/mco2.71080

Figure Lengend Snippet: Knockdown of CISD1 ameliorates BLM‐induced pulmonary fibrosis in mice. (A) Following AAV9‐mediated knockdown of CISD1 in mice, pulmonary fibrosis was induced using BLM. The pulmonary function of mice in each group was assessed using PFT, n = 6 biological replicates. (B–D) MicroCT analysis of the extent of lung injury in each group of mice; 3D reconstruction of mouse lungs was performed and the ratio of normally aerated lung tissue was quantified, n = 3–6 biological replicates, scale bar = 2 mm. (E and F). WB detection of fibrosis‐related protein expression in lung tissues from each group of mice, n = 6 biological replicates.

Article Snippet: Note that 21 days post intratracheal administration of AAV9 virus (PackGene Biotech, Guangzhou, China) containing shNC or shCISD1, a BLM‐induced pulmonary fibrosis model was established, and the mice were sacrificed after an additional 21 days of housing.

Techniques: Knockdown, Analysis, Tissue, Expressing